| Division 1 |  Colorado Rapids | |  Carolina RailHawks | | | Neutral Venue | |
| Division 1 |  Columbus Crew | |  Kansas City Wizards | | | Neutral Venue | |
| Division 1 |  Houston Dynamo | |  San Jose Earthquakes | | | Neutral Venue | |
| Division 1 |  Portland Timbers | |  Vancouver Whitecaps | | | Neutral Venue | |
| Division 2 |  Charleston Battery | |  Atlanta United | | | Neutral Venue | |
| Division 2 |  Austin FC | |  D.C. United | | | Neutral Venue | |
| | Division 2 |  Chivas USA | |  Los Angeles FC | | | Neutral Venue | |
| Division 2 |  Inter Miami | |  Seattle Sounders | | | Neutral Venue | |
| Division 3 |  De Anza Force S.C. | |  Carson City Kickers | | | Neutral Venue | |
| Division 3 |  Carolina Dynamo | |  KC Athletics | | | Neutral Venue | |
| Division 3 |  Daniel Island S.A. | |  Orange County SC | | | Neutral Venue | |
| | Division 3 |  Mount Tabor Portland | |  Columbus Rebels | | | Neutral Venue | |
| Division 4 |  Fort Lauderdale FC | |  Colorado Ice White | | | Neutral Venue | |
| Division 4 |  D.C. Colts | |  North Sound Seawolves | | | Neutral Venue | |
| Division 4 |  Laredo Heat | |  Savannah Celtic FC | | | Neutral Venue | |
| Division 4 |  San Antonio FC | |  Vancouver FC | | | Neutral Venue | |
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