| Division 1 | |  Colorado Rapids | |  Carolina RailHawks | | Dicks Sporting Goods Park | | |
| Division 1 | |  Columbus Crew | |  Kansas City Wizards | | Columbus Crew Stadium | | |
| Division 1 | |  Houston Dynamo | |  San Jose Earthquakes | | Robertson Stadium | | |
| Division 1 | |  Portland Timbers | |  Vancouver Whitecaps | | PGE Park | | |
| Division 2 | |  Charleston Battery | |  Atlanta United | | Blackbaud Stadium | | |
| Division 2 | |  Austin FC | |  D.C. United | | Q2 Stadium | | |
| | Division 2 | |  Chivas USA | |  Los Angeles FC | | The Home Depot Centre | | |
| Division 2 | |  Inter Miami | |  Seattle Sounders | | Chase Stadium | | |
| Division 3 | |  De Anza Force S.C. | |  Carson City Kickers | | Buck Shaw Stadium | | |
| Division 3 | |  Carolina Dynamo | |  KC Athletics | | Wakemed Soccer Park | | |
| Division 3 | |  Daniel Island S.A. | |  Orange County SC | | Blackbaud Stadium | | |
| | Division 3 | |  Mount Tabor Portland | |  Columbus Rebels | | Portland Park | | |
| Division 4 | |  Fort Lauderdale FC | |  Colorado Ice White | | Chase Stadium | | |
| Division 4 | |  D.C. Colts |  |  North Sound Seawolves | | RFK Stadium | | |
| Division 4 | |  Laredo Heat | |  Savannah Celtic FC | | Robertson Stadium | | |
| Division 4 | |  San Antonio FC | |  Vancouver FC | | Toyota Field | | |
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