| Division 1 | |  Carolina RailHawks | |  Portland Timbers | | Wakemed Soccer Park | | |
| Division 1 | |  Houston Dynamo | |  Vancouver Whitecaps | | Robertson Stadium | | |
| Division 1 | |  Columbus Crew | |  San Jose Earthquakes | | Columbus Crew Stadium | | |
| Division 1 | |  Colorado Rapids | |  Kansas City Wizards | | Dicks Sporting Goods Park | | |
| Division 2 | |  Atlanta United | |  Inter Miami | | ReMax Greater Atlanta Stadium | | |
| Division 2 | |  Chivas USA | |  Seattle Sounders | | The Home Depot Centre | | |
| | Division 2 | |  Austin FC | |  Los Angeles FC | | Q2 Stadium | | |
| Division 2 | |  Charleston Battery | |  D.C. United | | Blackbaud Stadium | | |
| Division 3 | |  Carson City Kickers | |  Mount Tabor Portland | | The Home Depot Centre | | |
| Division 3 | |  Daniel Island S.A. | |  Columbus Rebels | | Blackbaud Stadium | | |
| Division 3 | |  Carolina Dynamo | |  Orange County SC | | Wakemed Soccer Park | | |
| | Division 3 | |  De Anza Force S.C. | |  KC Athletics | | Buck Shaw Stadium | | |
| Division 4 | |  Colorado Ice White | |  San Antonio FC | | Dicks Sporting Goods Park | | |
| Division 4 | |  Laredo Heat | |  Vancouver FC | | Robertson Stadium | | |
| Division 4 | |  D.C. Colts | |  Savannah Celtic FC | | RFK Stadium | | |
| Division 4 | |  Fort Lauderdale FC | |  North Sound Seawolves | | Chase Stadium | | |
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