| Division 1 |  |  Atlanta United | |  Columbus Crew | | Neutral Venue | | |
| Division 1 | |  Austin FC | |  Kansas City Wizards | | Neutral Venue | | |
| Division 1 | |  Carolina RailHawks | |  Portland Timbers | | Neutral Venue | | |
| Division 1 | |  D.C. United | |  Los Angeles FC | | Neutral Venue | | |
| Division 2 | |  Charleston Battery | |  Houston Dynamo | | Neutral Venue | | |
| Division 2 | |  Chivas USA | |  Seattle Sounders | | Neutral Venue | | |
| | Division 2 | |  Colorado Rapids | |  Vancouver Whitecaps | | Neutral Venue | | |
| Division 2 | |  Inter Miami | |  San Jose Earthquakes | | Neutral Venue | | |
| Division 3 | |  Carolina Dynamo | |  Daniel Island S.A. | | Neutral Venue | | |
| Division 3 | |  Colorado Ice White | |  Mount Tabor Portland | | Neutral Venue | | |
| Division 3 | |  De Anza Force S.C. | |  Savannah Celtic FC | | Neutral Venue | | |
| | Division 3 | |  Fort Lauderdale FC | |  Columbus Rebels | | Neutral Venue | | |
| Division 4 | |  Carson City Kickers | |  Laredo Heat | | Neutral Venue | | |
| Division 4 | |  D.C. Colts | |  Orange County SC | | Neutral Venue | | |
| Division 4 | |  KC Athletics | |  Vancouver FC | | Neutral Venue | | |
| Division 4 | |  North Sound Seawolves | |  San Antonio FC | | Neutral Venue | | |
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