| Division 1 |  San Jose Earthquakes | |  Carolina RailHawks | | | Neutral Venue | |
| Division 1 |  Kansas City Wizards | |  Vancouver Whitecaps | | | Neutral Venue | |
| Division 1 |  Colorado Rapids | |  Portland Timbers | | | Neutral Venue | |
| Division 1 |  Columbus Crew | |  Houston Dynamo | | | Neutral Venue | |
| Division 2 |  Los Angeles FC | |  Atlanta United | | | Neutral Venue | |
| Division 2 |  D.C. United | |  Seattle Sounders | | | Neutral Venue | |
| | Division 2 |  Charleston Battery | |  Inter Miami | | | Neutral Venue | |
| Division 2 |  Austin FC | |  Chivas USA | | | Neutral Venue | |
| Division 3 |  Orange County SC | |  Carson City Kickers | | | Neutral Venue | |
| Division 3 |  KC Athletics | |  Columbus Rebels | | | Neutral Venue | |
| Division 3 |  De Anza Force S.C. | |  Mount Tabor Portland | | | Neutral Venue | |
| | Division 3 |  Carolina Dynamo | |  Daniel Island S.A. | | | Neutral Venue | |
| Division 4 |  Savannah Celtic FC | |  Colorado Ice White | | | Neutral Venue | |
| Division 4 |  North Sound Seawolves | |  Vancouver FC | | | Neutral Venue | |
| Division 4 |  Fort Lauderdale FC | |  San Antonio FC | | | Neutral Venue | |
| Division 4 |  D.C. Colts | |  Laredo Heat | | | Neutral Venue | |
|