| Division 1 | |  Portland Timbers | |  Austin FC | | Neutral Venue | | |
| Division 1 | |  Inter Miami | |  San Jose Earthquakes | | Neutral Venue | | |
| Division 1 | |  Columbus Crew | |  Kansas City Wizards | | Neutral Venue | | |
| Division 1 | |  Carolina RailHawks | |  Houston Dynamo | | Neutral Venue | | |
| Division 2 | |  Seattle Sounders | |  Atlanta United | | Neutral Venue | | |
| Division 2 | |  D.C. United | |  Vancouver Whitecaps | | Neutral Venue | | |
| | Division 2 | |  Chivas USA | |  Los Angeles FC | | Neutral Venue | | |
| Division 2 | |  Charleston Battery | |  Colorado Rapids | | Neutral Venue | | |
| Division 3 | |  San Antonio FC | |  Carolina Dynamo | | Neutral Venue | | |
| Division 3 | |  Orange County SC | |  Savannah Celtic FC | | Neutral Venue | | |
| Division 3 | |  Daniel Island S.A. | |  Columbus Rebels | | Neutral Venue | | |
| | Division 3 | |  De Anza Force S.C. | |  Mount Tabor Portland | | Neutral Venue | | |
| Division 4 | |  North Sound Seawolves | |  Carson City Kickers | | Neutral Venue | | |
| Division 4 | |  KC Athletics | |  Vancouver FC | | Neutral Venue | | |
| Division 4 | |  D.C. Colts | |  Laredo Heat | | Neutral Venue | | |
| Division 4 | |  Colorado Ice White | |  Fort Lauderdale FC | | Neutral Venue | | |
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