| Division 1 |  Austin FC | |  Inter Miami | | | Neutral Venue | |
| Division 1 |  Portland Timbers | |  Columbus Crew | | | Neutral Venue | |
| Division 1 |  San Jose Earthquakes | |  Carolina RailHawks | | | Neutral Venue | |
| Division 1 |  Kansas City Wizards | |  Houston Dynamo | | | Neutral Venue | |
| Division 2 |  Atlanta United | |  D.C. United | | | Neutral Venue | |
| Division 2 |  Seattle Sounders | |  Chivas USA | | | Neutral Venue | |
| | Division 2 |  Vancouver Whitecaps | |  Charleston Battery | | | Neutral Venue | |
| Division 2 |  Los Angeles FC | |  Colorado Rapids | | | Neutral Venue | |
| Division 3 |  Carolina Dynamo | |  Orange County SC | | | Neutral Venue | |
| Division 3 |  San Antonio FC | |  Daniel Island S.A. | | | Neutral Venue | |
| Division 3 |  Savannah Celtic FC | |  De Anza Force S.C. | | | Neutral Venue | |
| | Division 3 |  Columbus Rebels | |  Mount Tabor Portland | | | Neutral Venue | |
| Division 4 |  Carson City Kickers | |  KC Athletics | | | Neutral Venue | |
| Division 4 |  North Sound Seawolves | |  D.C. Colts | | | Neutral Venue | |
| Division 4 |  Vancouver FC | |  Colorado Ice White | | | Neutral Venue | |
| Division 4 |  Laredo Heat | |  Fort Lauderdale FC | | | Neutral Venue | |
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